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donkey serum d9663  (Millipore)


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    Structured Review

    Millipore donkey serum d9663
    Donkey Serum D9663, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/donkey+serum+d9663/triton+x+100/pm40553926-75-13-16
    Average 90 stars, based on 1 article reviews
    donkey serum d9663 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: A critical role for the Fascin family of actin bundling proteins in axon development, brain wiring and function.
    Article Snippet: .. The cells were blocked for one hr. at room temperature in 2 % donkey serum (D9663, Millipore-Sigma) in PBS, and then incubated overnight at 4 ◦C with mouse anti-tau (Sigma MAB3420, clone PC1C6) and rabbit anti-tubulin (Covance, PRB–435P) antibodies at 1:1000 in blocking buffer. .. Coverslips were next incubated in Alexa Fluorconjugated secondary antibodies (Invitrogen A10037 and A11036) diluted in PBS 1:500 for an hr. at room temperature.

    Article Title: DISRUPTING MECHANICAL HOMEOSTASIS PROMOTES MATRIX METALLOPROTEINASE-13 MEDIATED PROCESSING OF NEURON GLIAL ANTIGEN 2 IN MANDIBULAR CONDYLAR CARTILAGE
    Article Snippet: .. For immunohistochemistry, sections were dewaxed, treated with 132.2 mmol/L sodium borohydride, permeabilised with methanol and 0.5 % Triton (v/v), blocked in 5 % donkey serum (D9663, Sigma-Aldrich, St. Louis, MO, USA) for 2 h, and incubated with primary antibodies against either MMP-13 (1:200, AB39012, Abcam, Branford, CT, USA) or NG2/CSPG4 ectodomain (1:200, AB5320, Sigma-Millipore, Santa Cruz, CA, USA). .. All secondary labelling was with Alexa Fluor donkey anti-mouse 488 and donkey anti-rabbit 568 (1:500, Invitrogen, Invitrogen, Carlsbad, CA, USA).

    Article Title: A Critical Role for the Fascin Family of Actin Bundling Proteins in Axon Development, Brain Wiring and Function
    Article Snippet: .. The cells were blocked for one hr. at room temperature in 2% donkey serum (D9663, Millipore-Sigma) in PBS, and then incubated overnight at 4°C with mouse anti-tau (Sigma MAB3420, clone PC1C6) and rabbit anti-tubulin (Covance, PRB-435P) antibodies at 1:1000 in blocking buffer. .. Coverslips were next incubated in Alexa Fluor-conjugated secondary antibodies (Invitrogen A10037 and A11036) diluted in PBS 1:500 for an hr. at room temperature.

    Article Title: ROR2 regulates cellular plasticity in pancreatic neoplasia and adenocarcinoma
    Article Snippet: Cells were fixed with 4% formaldehyde for 10 minutes at RT, washed with PBS and permeabilized using PBS containing 0.25% Triton X-100 (T8787, Millipore Sigma). .. After blocking in PBS supplemented with 1% bovine serum albumin (BSA) (BP9703, Thermo Fisher Scientific), 5% donkey serum (D9663, Millipore Sigma) and 0.02% Triton X-100, primary antibodies were incubated overnight at 4°C. ..

    Blocking Assay:

    Article Title: A critical role for the Fascin family of actin bundling proteins in axon development, brain wiring and function.
    Article Snippet: .. The cells were blocked for one hr. at room temperature in 2 % donkey serum (D9663, Millipore-Sigma) in PBS, and then incubated overnight at 4 ◦C with mouse anti-tau (Sigma MAB3420, clone PC1C6) and rabbit anti-tubulin (Covance, PRB–435P) antibodies at 1:1000 in blocking buffer. .. Coverslips were next incubated in Alexa Fluorconjugated secondary antibodies (Invitrogen A10037 and A11036) diluted in PBS 1:500 for an hr. at room temperature.

    Article Title: Functional neurological restoration of amputated peripheral nerve using biohybrid regenerative bioelectronics.
    Article Snippet: .. Immunofluorescence (Fig. 2, F and G) is carried out by performing antigen retrieval in citrate buffer for 20 min followed by blocking samples in a PBS solution of 5% donkey serum (D9663, SigmaAldrich) and 0.1% sodium azide at room temperature of 1 hour. .. Samples are then stained with anti–b3-tubulin (1/1000 dilution in blocking medium; ab7751, Abcam) and anti–acetylcholinesterase (AChE) (1/100 dilution; ab183591, Abcam).

    Article Title: A Critical Role for the Fascin Family of Actin Bundling Proteins in Axon Development, Brain Wiring and Function
    Article Snippet: .. The cells were blocked for one hr. at room temperature in 2% donkey serum (D9663, Millipore-Sigma) in PBS, and then incubated overnight at 4°C with mouse anti-tau (Sigma MAB3420, clone PC1C6) and rabbit anti-tubulin (Covance, PRB-435P) antibodies at 1:1000 in blocking buffer. .. Coverslips were next incubated in Alexa Fluor-conjugated secondary antibodies (Invitrogen A10037 and A11036) diluted in PBS 1:500 for an hr. at room temperature.

    Article Title: ROR2 regulates cellular plasticity in pancreatic neoplasia and adenocarcinoma
    Article Snippet: Cells were fixed with 4% formaldehyde for 10 minutes at RT, washed with PBS and permeabilized using PBS containing 0.25% Triton X-100 (T8787, Millipore Sigma). .. After blocking in PBS supplemented with 1% bovine serum albumin (BSA) (BP9703, Thermo Fisher Scientific), 5% donkey serum (D9663, Millipore Sigma) and 0.02% Triton X-100, primary antibodies were incubated overnight at 4°C. ..

    Article Title: Lipid-siRNA conjugate accesses a perivascular transport mechanism and achieves widespread and durable knockdown in the central nervous system
    Article Snippet: .. Samples were washed in DPBS-/- with 0.3% Triton-X 100 (PBST), followed by blocking for 1 hour at room temperature in PBST plus 5% donkey serum (Sigma D9663). ..

    Immunohistochemistry:

    Article Title: DISRUPTING MECHANICAL HOMEOSTASIS PROMOTES MATRIX METALLOPROTEINASE-13 MEDIATED PROCESSING OF NEURON GLIAL ANTIGEN 2 IN MANDIBULAR CONDYLAR CARTILAGE
    Article Snippet: .. For immunohistochemistry, sections were dewaxed, treated with 132.2 mmol/L sodium borohydride, permeabilised with methanol and 0.5 % Triton (v/v), blocked in 5 % donkey serum (D9663, Sigma-Aldrich, St. Louis, MO, USA) for 2 h, and incubated with primary antibodies against either MMP-13 (1:200, AB39012, Abcam, Branford, CT, USA) or NG2/CSPG4 ectodomain (1:200, AB5320, Sigma-Millipore, Santa Cruz, CA, USA). .. All secondary labelling was with Alexa Fluor donkey anti-mouse 488 and donkey anti-rabbit 568 (1:500, Invitrogen, Invitrogen, Carlsbad, CA, USA).

    Immunofluorescence:

    Article Title: Functional neurological restoration of amputated peripheral nerve using biohybrid regenerative bioelectronics.
    Article Snippet: .. Immunofluorescence (Fig. 2, F and G) is carried out by performing antigen retrieval in citrate buffer for 20 min followed by blocking samples in a PBS solution of 5% donkey serum (D9663, SigmaAldrich) and 0.1% sodium azide at room temperature of 1 hour. .. Samples are then stained with anti–b3-tubulin (1/1000 dilution in blocking medium; ab7751, Abcam) and anti–acetylcholinesterase (AChE) (1/100 dilution; ab183591, Abcam).



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